| First Author | Hildebrand DG | Year | 2013 |
| Journal | J Immunol | Volume | 190 |
| Issue | 9 | Pages | 4812-20 |
| PubMed ID | 23547114 | Mgi Jnum | J:195500 |
| Mgi Id | MGI:5484693 | Doi | 10.4049/jimmunol.1300089 |
| Citation | Hildebrand DG, et al. (2013) IkappaBzeta Is a Transcriptional Key Regulator of CCL2/MCP-1. J Immunol 190(9):4812-20 |
| abstractText | CCL2, also referred to as MCP-1, is critically involved in directing the migration of blood monocytes to sites of inflammation. Consequently, excessive CCL2 secretion has been linked to many inflammatory diseases, whereas a lack of expression severely impairs immune responsiveness. We demonstrate that IkappaBzeta, an atypical IkappaB family member and transcriptional coactivator required for the selective expression of a subset of NF-kappaB target genes, is a key activator of the Ccl2 gene. IkappaBzeta-deficient macrophages exhibited impaired secretion of CCL2 when challenged with diverse inflammatory stimuli, such as LPS or peptidoglycan. These findings were reflected at the level of Ccl2 gene expression, which was tightly coupled to the presence of IkappaBzeta. Moreover, mechanistic insights acquired by chromatin immunoprecipitation demonstrate that IkappaBzeta is directly recruited to the proximal promoter region of the Ccl2 gene and is required for transcription-enhancing histone H3 at lysine-4 trimethylation. Finally, IkappaBzeta-deficient mice showed significantly impaired CCL2 secretion and monocyte infiltration in an experimental model of peritonitis. Together, these findings suggest a distinguished role of IkappaBzeta in mediating the targeted recruitment of monocytes in response to local inflammatory events. |
