| First Author | He Y | Year | 2017 |
| Journal | Genesis | Volume | 55 |
| Issue | 9 | PubMed ID | 28722198 |
| Mgi Jnum | J:245518 | Mgi Id | MGI:5916301 |
| Doi | 10.1002/dvg.23048 | Citation | He Y, et al. (2017) Male germline recombination of a conditional allele by the widely used Dermo1-cre (Twist2-cre) transgene. Genesis 55(9) |
| abstractText | Conditional gene knockout using the Cre/loxP system is instrumental in advancing our understanding of the function of genes in a wide range of disciplines. It is becoming increasingly apparent in the literature that recombination mediated by some Cre transgenes can occur in unexpected tissues. Dermo1-Cre (Twist2-Cre) has been widely used to target skeletal lineage cells as well as other mesoderm-derived cells. Here we report that Dermo1-Cre exhibits spontaneous male germline recombination activity leading to a Cre-mediated recombination of a floxed Ptk2 (Protein tyrosine kinase 2, also known as Fak [Focal adhesion kinase]) allele but not a floxed Rb1cc1 (RB1 inducible coiled-coil 1, also known as Fip200 [FAK-family Interacting Protein of 200 kDa]) allele at high frequency. This ectopic germline activity of Dermo1-Cre occurred in all or none manner in a given litter. We demonstrated that the occurrence of germline recombination activity of Dermo1-Cre transgene can be avoided by using female mice as parental Dermo1-Cre carriers. |
