| Primary Identifier | MGI:3526430 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nherf1 |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting vector was designed to replace exons 1-4, which contain most of the coding sequence, with a neo gene lacking the polyadenlylation sequence. RT-PCR and sequencing revealed that exons 5 and 6 were transcribed after the stop codon of the neo mRNA, as part of the 3' UTR. Absence of protein from exons 5 and 6 was further confirmed by Western blot analysis of kidneys from mutant animals. |
