Primary Identifier | MGI:3526438 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Ihh |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Frt-flanked neo-tk cassette with a loxP site was introduced into the first intron and another loxP site was introduced into the 5' leader sequence of the first exon by homologous recombination. The neo-tk cassette was excised by transient transfection of properly targeted ES cells with a flpe-expressing plasmid, leaving exon 1 flanked by loxP sites. |