| Primary Identifier | MGI:3526658 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rdh11 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Targeted disruption of RDH11 resulted in the replacement of exons 2 and 3 with the neomycin gene. Exon 2 contains the NADPH binding site. Any resulting transcript from exon 1 would be 22 amino acids long. Alternative splicing from this exon to any of exons 4-7 would cause a frameshift resulting in a premature stop codon. Immunoblotting demonstrated the loss of protein from mutant testes. Transcript was not found in mutant liver tissue. |
