| Primary Identifier | MGI:3528226 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Card11 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = GS (129S6/SvEvTac). |
| molecularNote | Exons 2 and 3, encoding the caspase activation and recruitment domain (CARD) were replaced with a lacZ reporter gene and a PGK-neo. Retention of stop codons downstream of the exon 2 splice acceptor site and a consensus Kozak start site for lacZ, as well as the splicing of exon 1 to exon 4 allowed an ORF that included lacZ. Immunoprecipitation and Western blot of mutant spleen cells confirmed production of a protein without the CARD. Beta-galactosidase activity was detected in thymus, spleen and lymph nodes, but negligible in bone marrow of heterozygous mice. |
