Primary Identifier | MGI:3531175 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Pou5f1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | One loxP site was inserted upstream of exon1 and the essential promoter. A floxed neo cassette was inserted into intron 1. Exon 1 and the selection cassette were excised by subsequent transient transcription of Cre recombinase in the ES cells. Northern blot analysis did not detect gene product mRNA. |