| Primary Identifier | MGI:3530910 | Allele Type | Gene trapped |
| Attribute String | Hypomorph | Gene | Pknox1 |
| Transmission | Germline | Strain of Origin | 129S5/SvEvBrd |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Lexicon |
| molecularNote | The clone used for gene trapping was isolated from a library of embryonic stem cells randomly targeted with the retroviral vector VICTR45 using an enhancer trap strategy. Retroviral insertion occurred at nucleotide 849 of intron 1 located within the 5' untranslated region (2.8 kb from the ATG). RT-PCR analysis revealed that transcript was absent in most homozygous mice. However, in some homozygotes a low level of protein (about 5-10% of wild-type) is detected. |
