Primary Identifier | MGI:3575088 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Tnfrsf4 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was created to introduce a stop codon at amino acid 150. A neomycin resistance cassette was also included in the vector for selection purposes. Absence of transcript was confirmed by RT-PCR of total RNA from mutants. Flow cytometry further showed absence of protein on the surface of stimulated mutant splenocytes. |