| Primary Identifier | MGI:3577345 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Polh |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | LoxP sites were introduced to flank exon 4, which contains the DE polymerase motifs conserved in all Y family polymerases. Cre expression mediated excision of exon 4 in ES cells, resulting in the direct splicing of exons 3-5. The out-of-frame junction would introduce a stop codon and therefore a truncated protein of 102 amino acids. |
