Primary Identifier | MGI:3579250 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Cbfb |
Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
Is Recombinase | false | Is Wild Type | false |
molecularNote | ES cells containing Cbfbtm1Ppl were targeted a second time to insert a floxed cassette encompassing exons 5 and 6, as well as the bovine growth hormone polyA signal and a PGK-hygro cassette into the Msc1 site in intron 4. This design allows the wild-type Cbfb transcript to be expressed. Cre expression would remove the cassette and restore expression of the knockin allele. |