| Primary Identifier | MGI:3583076 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(Ren1-GFP)1Kwg |
| Strain of Origin | (C57BL/10-Oca2<p-d>/Ros x C3H/HeRos)F1 | Is Recombinase | false |
| Is Wild Type | false |
| description | Transgenic mice express green fluorescent protein (GFP) in juxtaglomerular (JG) cells of the kidney and in granular convoluted tubular cells of the submandibular gland (SMG). Treatment of mice with the ACE inhibitor captopril induces GFP expression in renal vascular smooth muscle cells. In the SMG, GFP expression is androgen responsive. GFP expression is first detected at embryonic day E13 in the adrenal gland and Wolffian duct, by E14 is also seen in the developing renal vasculature, and remains detectable through birth. In adults, renal GFP expression becomes restricted to JG cells. Fetal adrenal and gonadal arteries also express GFP. In the placenta, GFP is observed in giant cell trophoblasts. |
| molecularNote | The 5.2-kb transgene comprises a "humanized" green fluorescent protein (GFP) coding sequence, flanked by the splice donor/acceptor sites and polyadenylation signal from SV40, downstream of approximately 4.1 kb of mouse genomic DNA containing the Ren1 5' flanking promoter/enhancer. Six to seven copies of the transgene were estimated by semi-quantitative Southern blot analysis to be present per haploid genome. Combined SKY-FISH analysis localized the single integration site to Chromosome 4. |
