| Primary Identifier | MGI:3586923 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Ube2d2a |
| Transmission | Germline | Strain of Origin | Not Specified |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = RS1. |
| molecularNote | Sequence encoding amino acid residues 2 to 126 were replaced with a neomycin resistance cassette, removing 124 of 147 residues, including all of the elements making up the active site. Northern blot confirmed absence of wild-type transcript in mutant testis, but showed the presence of a longer transcript resulting from transcription through the larger neomycin resistance cassette. Western blot demonstrated absence of protein in mutant testis. |
