| First Author | Chung HJ | Year | 2003 |
| Journal | Science | Volume | 300 |
| Issue | 5626 | Pages | 1751-5 |
| PubMed ID | 12805550 | Mgi Jnum | J:107862 |
| Mgi Id | MGI:3622391 | Doi | 10.1126/science.1082915 |
| Citation | Chung HJ, et al. (2003) Requirement of AMPA receptor GluR2 phosphorylation for cerebellar long-term depression. Science 300(5626):1751-5 |
| abstractText | Cerebellar long-term depression (LTD) is a model of synaptic memory that requires protein kinase C (PKC) activation and is expressed as a reduction in the number of postsynaptic alpha-amino-3-hydroxy-5-methyl-4-isoxazolepropionate (AMPA) receptors. LTD was absent in cultured cerebellar Purkinje cells from mutant mice lacking the AMPA receptor GluR2 subunit and could be rescued by transient transfection with the wild-type GluR2 subunit. Transfection with a point mutant that eliminated PKC phosphorylation of Ser880 in the carboxy-terminal PDZ ligand of GluR2 failed to restore LTD. In contrast, transfection with a point mutant that mimicked phosphorylation at Ser880 occluded subsequent LTD. Thus, PKC phosphorylation of GluR2 Ser880 is a critical event in the induction of cerebellar LTD. |
