| Primary Identifier | MGI:3586582 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(TcraCl4,TcrbCl4)1Shrm |
| Strain of Origin | (C57BL/6 x BALB/c)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Tcra and Tcrb cDNAs were isolated from Clone-4, a CTL line derived from a B10.D2-Hc1 H2d H2-t18c/nSnJ mouse immunized with influenza virus A/PR/8. A Tcra cDNA fragment representing the V-J exon, containing Valpha10, was cloned into a Tcra shuttle vector that includes a mouse Tcra-V promoter, the immunoglobulin heavy-chain enhancer EH, and the Tcra-C genes with some flanking DNA. Likewise, a Tcrb cDNA fragment representing the VDJ exon, containing Vbeta8.2, was cloned into a Tcrb shuttle vector including a mouse Tcrb-V promoter, EH, and the Tcrb-C genes with some flanking DNA. The plasmid constructs were coinjected. This transgenic TCR specifically recognizes influenza virus A/PR/8 hemagglutinin restricted by MHC class I H2kd. |
