| Primary Identifier | MGI:3588425 | Allele Type | Targeted |
| Attribute String | Null/knockout, Reporter | Gene | Onecut2 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to insert a lacZ-puro cassette into exon 1, deleting sequence that encodes transcriptional regulatory regions and for the CUT domain required for DNA binding. In-frame stop codons were included in the vector, following the lacZ sequence to prevent the production of a truncated Onecut2 protein or read-through transcripts initiated in the lacZ or puro cassettes. RT-PCR failed to detect Onecut2 transcript in mutant liver samples. |
