| First Author | Hornstein E | Year | 2005 |
| Journal | Nature | Volume | 438 |
| Issue | 7068 | Pages | 671-4 |
| PubMed ID | 16319892 | Mgi Jnum | J:103424 |
| Mgi Id | MGI:3609457 | Doi | 10.1038/nature04138 |
| Citation | Hornstein E, et al. (2005) The microRNA miR-196 acts upstream of Hoxb8 and Shh in limb development. Nature 438(7068):671-4 |
| abstractText | MicroRNAs (miRNAs) are an abundant class of gene regulatory molecules (reviewed in refs 1, 2). Although computational work indicates that miRNAs repress more than a third of human genes, their roles in vertebrate development are only now beginning to be determined. Here we show that miR-196 acts upstream of Hoxb8 and Sonic hedgehog (Shh) in vivo in the context of limb development, thereby identifying a previously observed but uncharacterized inhibitory activity that operates specifically in the hindlimb. Our data indicate that miR-196 functions in a fail-safe mechanism to assure the fidelity of expression domains that are primarily regulated at the transcriptional level, supporting the idea that many vertebrate miRNAs may function as a secondary level of gene regulation. |
