| First Author | Shimizu T | Year | 2023 |
| Journal | Sci Rep | Volume | 13 |
| Issue | 1 | Pages | 4271 |
| PubMed ID | 36922541 | Mgi Jnum | J:334820 |
| Mgi Id | MGI:7445995 | Doi | 10.1038/s41598-023-31322-3 |
| Citation | Shimizu T, et al. (2023) Polycystic kidney disease 2-like 1 channel contributes to the bitter aftertaste perception of quinine. Sci Rep 13(1):4271 |
| abstractText | Bitterness is an important physiological function in the defense responses to avoid toxic foods. The taste receptor 2 family is well known to mediate bitter taste perception in Type II taste cells. Here, we report that the polycystic kidney disease 2-like 1 (PKD2L1) channel is a novel sensor for the bitter aftertaste in Type III taste cells. The PKD2L1 channel showed rebound activation after the washout of quinine, a bitter tastant, in electrophysiological whole-cell recordings of the PKD2L1-expressing HEK293T cells and Ca(2+)-imaging analysis of Type III taste cells isolated from wild-type PKD2L1 mice. In the short-term two-bottle preference and lick tests in vivo, the wild-type mice avoided normal water while the PKD2L1-knockout mice preferred normal water after they ingested the quinine-containing water. These results may explain the new mechanism of the quinine-triggered bitter aftertaste perception in Type III taste cells. |
