| Primary Identifier | MGI:3589287 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Dicer1 |
| Transmission | Germline | Strain of Origin | B6.Cg-Thy1<a> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | LoxP sites were inserted to flank exons 18-20, which encode part of the first RNase III domain. Deletion of this sequence putatively leads to out-of-frame splicing of all downstream exons, including the second RNase III and the dsRNA-binding domains, and results in complete ablation of enzymactic activity. An frt-flanked neo was inserted into intron 17 and was removed by crossing mice with a strain that expresses Flp recombinase in the germ line. |
