| Primary Identifier | MGI:3590082 | Allele Type | Transgenic |
| Attribute String | Humanized sequence, Inserted expressed sequence | Gene | Tg(CD2-CD4,HLA-DQA1,HLA-DQB1)1Ell |
| Strain of Origin | NOD/MrkTac | Is Recombinase | false |
| Is Wild Type | false |
| description | Although in NOD mice homozygous for this transgene alone endogeous NOD H2-Abetag7 and transgene-derived HLA-DQ8 are expressed at similar levels, virtually all CD4+ T-cell responses are H2Abeta1g7 restricted (J:87013).
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| molecularNote | Fertilized oocytes were co-injected with two DNA fragments: a human CD4 cDNA joined to the promoter from the human CD2 gene and a large DNA fragment, reassembled from segments of the cosmid pDCbeta2, containing the human major histocompatibility (MHC) class II alleles HLA-DQA1*0301 and HLA-DQB*0302, which together encode the dimer comprising the HLA-DQ8 molecule, plus 5' regulatory elements. Human CD4 is expressed at a low level on all T cells of transgenic mice. HLA-DQ8 is expressed in homozygous NOD mice at a level comparable to that of the endogenous NOD H2-Abetag7 MHC class II molecule in wild-type NOD mice. |
