| First Author | Nam HS | Year | 2009 |
| Journal | Cell Stem Cell | Volume | 5 |
| Issue | 5 | Pages | 515-26 |
| PubMed ID | 19896442 | Mgi Jnum | J:154023 |
| Mgi Id | MGI:4366897 | Doi | 10.1016/j.stem.2009.08.017 |
| Citation | Nam HS, et al. (2009) High levels of Id1 expression define B1 type adult neural stem cells. Cell Stem Cell 5(5):515-26 |
| abstractText | Defining the molecular identity of stem cells may be critical for formulating a rational strategy for the therapeutic intervention of stem cell dysfunction. We find that high expression of Id1, a dominant-negative helix-loop-helix transcriptional regulator, identifies a rare population of GFAP(+) astrocytes with stem cell attributes among the subventricular astrocytes in the adult brain. The rare, long-lived, and relatively quiescent Id1(high) astrocytes with morphology characteristic of B1 type astrocytes self-renew and generate migratory neuroblasts that differentiate into olfactory bulb interneurons. Cultured Id1(high) neural stem cells can self-renew asymmetrically and generate a stem and a differentiated cell expressing progressively lower levels of Id1, revealing an Id1 gradient in unperturbed cells of subventricular neurogenic lineages. Moreover, Id genes are necessary to confer self-renewal capacity, a characteristic of stem cell identity. We suggest that high expression of a single transcriptional regulator, Id1, molecularly defines the long-sought-after B1 type adult neural stem cells. |
