| First Author | Villette V | Year | 2019 |
| Journal | Cell | Volume | 179 |
| Issue | 7 | Pages | 1590-1608.e23 |
| PubMed ID | 31835034 | Mgi Jnum | J:358686 |
| Mgi Id | MGI:6436784 | Doi | 10.1016/j.cell.2019.11.004 |
| Citation | Villette V, et al. (2019) Ultrafast Two-Photon Imaging of a High-Gain Voltage Indicator in Awake Behaving Mice. Cell 179(7):1590-1608.e23 |
| abstractText | Optical interrogation of voltage in deep brain locations with cellular resolution would be immensely useful for understanding how neuronal circuits process information. Here, we report ASAP3, a genetically encoded voltage indicator with 51% fluorescence modulation by physiological voltages, submillisecond activation kinetics, and full responsivity under two-photon excitation. We also introduce an ultrafast local volume excitation (ULoVE) method for kilohertz-rate two-photon sampling in vivo with increased stability and sensitivity. Combining a soma-targeted ASAP3 variant and ULoVE, we show single-trial tracking of spikes and subthreshold events for minutes in deep locations, with subcellular resolution and with repeated sampling over days. In the visual cortex, we use soma-targeted ASAP3 to illustrate cell-type-dependent subthreshold modulation by locomotion. Thus, ASAP3 and ULoVE enable high-speed optical recording of electrical activity in genetically defined neurons at deep locations during awake behavior. |
