| First Author | Somarowthu A | Year | 2021 |
| Journal | Cell Calcium | Volume | 96 |
| Pages | 102380 | PubMed ID | 33676317 |
| Mgi Jnum | J:333569 | Mgi Id | MGI:6728204 |
| Doi | 10.1016/j.ceca.2021.102380 | Citation | Somarowthu A, et al. (2021) Two-photon calcium imaging of seizures in awake, head-fixed mice. Cell Calcium 96:102380 |
| abstractText | Epilepsy is a severe neurological disorder defined by spontaneous seizures. Current treatment options fail in a large proportion of patients, while questions as to the basic mechanisms of seizure initiation and propagation remain. Advances in imaging of seizures in experimental model systems could lead to a better understanding of mechanisms of seizures and epilepsy. Recent studies have used two-photon calcium imaging (2P imaging) in awake, behaving mice in head-fixed preparations to image seizures in vivo at high speed and cellular-level resolution to identify key seizure-related cell classes. Here, we discuss such advances and present 2P imaging data of excitatory neurons and defined subsets of cerebral cortex GABAergic inhibitory interneurons during naturalistic seizures in a mouse model of Dravet syndrome (Scn1a+/- mice) along with other behavioral measures. Results demonstrate differential recruitment of discrete interneuron subclasses, which could inform mechanisms of seizure generation and propagation in Dravet syndrome and other epilepsies. |
