| First Author | Nguyen QA | Year | 2018 |
| Journal | Neuron | Volume | 98 |
| Issue | 4 | Pages | 718-725.e3 |
| PubMed ID | 29706582 | Mgi Jnum | J:269364 |
| Mgi Id | MGI:6268940 | Doi | 10.1016/j.neuron.2018.03.046 |
| Citation | Nguyen QA, et al. (2018) The GABAA Receptor beta Subunit Is Required for Inhibitory Transmission. Neuron 98(4):718-725.e3 |
| abstractText | While the canonical assembly of a GABAA receptor contains two alpha subunits, two beta subunits, and a fifth subunit, it is unclear which variants of each subunit are necessary for native receptors. We used CRISPR/Cas9 to dissect the role of the GABAA receptor beta subunits in inhibitory transmission onto hippocampal CA1 pyramidal cells and found that deletion of all beta subunits 1, 2, and 3 completely eliminated inhibitory responses. In addition, only knockout of beta3, alone or in combination with another beta subunit, impaired inhibitory synaptic transmission. We found that beta3 knockout impairs inhibitory input from PV but not SOM expressing interneurons. Furthermore, expression of beta3 alone on the background of the beta1-3 subunit knockout was sufficient to restore synaptic and extrasynaptic inhibitory transmission. These findings reveal a crucial role for the beta3 subunit in inhibitory transmission and identify a synapse-specific role of the beta3 subunit in GABAergic synaptic transmission. |
