| First Author | Wyroślak M | Year | 2023 |
| Journal | Front Cell Neurosci | Volume | 17 |
| Pages | 1193383 | PubMed ID | 37448697 |
| Mgi Jnum | J:346967 | Mgi Id | MGI:7507911 |
| Doi | 10.3389/fncel.2023.1193383 | Citation | Wyroslak M, et al. (2023) Bidirectional plasticity of GABAergic tonic inhibition in hippocampal somatostatin- and parvalbumin-containing interneurons. Front Cell Neurosci 17:1193383 |
| abstractText | GABA(A) receptors present in extrasynaptic areas mediate tonic inhibition in hippocampal neurons regulating the performance of neural networks. In this study, we investigated the effect of NMDA-induced plasticity on tonic inhibition in somatostatin- and parvalbumin-containing interneurons. Using pharmacological methods and transgenic mice (SST-Cre/PV-Cre x Ai14), we induced the plasticity of GABAergic transmission in somatostatin- and parvalbumin-containing interneurons by a brief (3 min) application of NMDA. In the whole-cell patch-clamp configuration, we measured tonic currents enhanced by specific agonists (etomidate or gaboxadol). Furthermore, in both the control and NMDA-treated groups, we examined to what extent these changes depend on the regulation of distinct subtypes of GABA(A) receptors. Tonic conductance in the somatostatin-containing (SST+) interneurons is enhanced after NMDA application, and the observed effect is associated with an increased content of alpha5-containing GABA(A)Rs. Both fast-spiking and non-fast-spiking parvalbumin-positive (PV+) cells showed a reduction of tonic inhibition after plasticity induction. This effect was accompanied in both PV+ interneuron types by a strongly reduced proportion of delta-subunit-containing GABA(A)Rs and a relatively small increase in currents mediated by alpha5-containing GABA(A)Rs. Both somatostatin- and parvalbumin-containing interneurons show cell type-dependent and opposite sign plasticity of tonic inhibition. The underlying mechanisms depend on the cell-specific balance of plastic changes in the contents of alpha5 and delta subunit-containing GABA(A)Rs. |
