Primary Identifier | MGI:6383454 | Allele Type | Targeted |
Attribute String | Conditional ready, Hypomorph, Reporter | Gene | Trpc5 |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted upstream of exon 4. A floxed cassette with a splice acceptor, IRES, and GFP reporter gene were inserted downstream of exon 4. Flp-mediated recombination removed the FRT-flanked neomycin resistance cassette previously located within the reporter cassette. Immunohistochemistry confirms the reduction in transcript expression in ARC neurons. |