| Primary Identifier | MGI:3604459 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Tcra-J |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Using ES cells with a single loxP site 5' of the endogenous TEA promoter, a targeting vector was used to insert a floxed neo, TEA promoter, TEA exon, Jalpha61 and Jalpha56 sequences downstream of the Jalpha region. Resultant ES cells were transfected with cre recombinase, leading to the 66-kb deletion between the loxP sites 5' of the TAE endogenous promoter and 3' of the neomycin resistant gene. Only the Jalpha56 could give rise to a functional Jalpha arrangement in mutant mice. These mice have increased thymocyytes but reduced mature alphabeta T cell numbers. |
