| Primary Identifier | MGI:3604485 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Gria4 |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| Project Collection | Deltagen |
| molecularNote | Genotyping (long range PCR and genomic sequencing) for this allele revealed that the 5' half of intron 3-lacZ-neo-intron 3 construct inserted into Gria4 intron 3 resulting in truncation of most of the lacZ gene and some of intron 3, instead of the intended homologous replacement of Gria4 exon 3 with a lacZ-neo cassette. No Gria4 RNA was detected in the brain by RT-PCR. |
