| Primary Identifier | MGI:3606973 | Allele Type | Radiation induced |
| Gene | Oc90 | Transmission | Germline |
| Strain of Origin | (C57BL/6J x 129X1/SvJ)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | An HSV-tk/beta-actin-neo cassette was inserted into intron 2 using a gene targeting strategy. ES cells expressing both markers were selected. After X-irradiation of properly targeted ES cell lines with 400 rad, loss of tk expression was determined using FIAV. Cells no longer expressing tk were further tested for loss of neomycin expression. SSLP typing of polymorphic microsatellite markers determined that this allele contains a deletion of probably less than 658 kb. FISH confirmed deletion of genomic sequence. |
