| Primary Identifier | MGI:3606979 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Spag16 |
| Transmission | Germline | Strain of Origin | 129S6/SvEvTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | A targeting construct was designed to replace exons 2 and 3 with a neomycin resistance cassette, thereby preventing expression of the 71-kDa protein, but allowing expression of the 35-kDa protein. Northern and immunoblot analyses confirmed absence of wild type transcript and protein in mutant. A truncated 0.5 kb mRNA was detected in mutant mice, a truncated protein corresponding to the long isoform was not detected. |
