| Primary Identifier | MGI:3609975 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Nt5e |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The floxed exon 2 and neomycin resistance gene were deleted from the Nt5etm2Jsc allele via cre-mediated excision. RT-PCR detected a shortened transcript at levels similar to wild-type. The mutation caused a frame shift at the new exon 1 to 3 junction, resulting in the termination of translation after the first exon. Protein was not detected in Western blot. |
