| First Author | Srivastava R | Year | 2018 |
| Journal | J Immunol | Volume | 201 |
| Issue | 8 | Pages | 2315-2330 |
| PubMed ID | 30201808 | Mgi Jnum | J:266197 |
| Mgi Id | MGI:6203270 | Doi | 10.4049/jimmunol.1800725 |
| Citation | Srivastava R, et al. (2018) Phenotypic and Functional Signatures of Herpes Simplex Virus-Specific Effector Memory CD73(+)CD45RA(high)CCR7(low)CD8(+) TEMRA and CD73(+)CD45RA(low)CCR7(low)CD8(+) TEM Cells Are Associated with Asymptomatic Ocular Herpes. J Immunol 201(8):2315-2330 |
| abstractText | HSV type 1 (HSV-1)-specific CD8(+) T cells protect from herpes infection and disease. However, the nature of protective CD8(+) T cells in HSV-1 seropositive healthy asymptomatic (ASYMP) individuals (with no history of clinical herpes disease) remains to be determined. In this study, we compared the phenotype and function of HSV-specific CD8(+) T cells from HLA-A*02:01-positive ASYMP and symptomatic (SYMP) individuals (with a documented history of numerous episodes of recurrent ocular herpetic disease). We report that although SYMP and ASYMP individuals have similar frequencies of HSV-specific CD8(+) T cells, the "naturally" protected ASYMP individuals have a significantly higher proportion of multifunctional HSV-specific effector memory CD8(+) T cells (CD73(+)CD45RA(high)CCR7(low)CD8(+) effector memory RA (TEMRA) and CD73(+)CD45RA(low)CCR7(low)CD8(+) effector memory (TEM) as compared with SYMP individuals. Similar to humans, HSV-1-infected ASYMP B6 mice had frequent multifunctional HSV-specific CD73(+)CD8(+) T cells in the cornea, as compared with SYMP mice. Moreover, in contrast to wild type B6, CD73(-/-) deficient mice infected ocularly with HSV-1 developed more recurrent corneal herpetic infection and disease. This was associated with less functional CD8(+) T cells in the cornea and trigeminal ganglia, the sites of acute and latent infection. The phenotypic and functional characteristics of HSV-specific circulating and in situ CD73(+)CD8(+) T cells, demonstrated in both ASYMP humans and mice, suggest a positive role for effector memory CD8(+) T cells expressing the CD73 costimulatory molecule in the protection against ocular herpes infection and disease. These findings are important for the development of safe and effective T cell-based herpes immunotherapy. |
