| Primary Identifier | MGI:3610301 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Wt1 |
| Transmission | Chimeric | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | ES cell line = E14 or CGR8. The ES cell line used to derive the one reported heterozygous male is not noted in J:53585 or J:103489. Phenotypic data described in these publications are for the single heterozygous male obtained from a female chimera. Chimeras exhibit a similar, although less severe, phenotype as the heterozygous male. |
| molecularNote | The insertion of a Leu2/neo cassette resulted in the S395R amino acid substitution and a premature termination within ZF3 by inclusion of a translational stop codon at 396. This would cause a truncation of 7.3 kDa with the loss of 52 amino acids, including the KTS insert and ZF4, and would render ZF3 nonfunctional by removing its alpha-helix. Western blot analysis confirmed truncation of two of the four isoforms. Cell fractionation showed that the low level of mutant protein was not a result of retention in the cytoplasm, therefore, posttranscriptional events must account for the fact that only 5% of the protein is mutant. |
