| First Author | Alam J | Year | 2022 |
| Journal | Front Med (Lausanne) | Volume | 9 |
| Pages | 849990 | PubMed ID | 35402439 |
| Mgi Jnum | J:361471 | Mgi Id | MGI:7857027 |
| Doi | 10.3389/fmed.2022.849990 | Citation | Alam J, et al. (2022) IL-17 Producing Lymphocytes Cause Dry Eye and Corneal Disease With Aging in RXRalpha Mutant Mouse. Front Med (Lausanne) 9:849990 |
| abstractText | PURPOSE: To investigate IL-17 related mechanisms for developing dry eye disease in the Pinkie mouse strain with a loss of function RXRalpha mutation. METHODS: Measures of dry eye disease were assessed in the cornea and conjunctiva. Expression profiling was performed by single-cell RNA sequencing (scRNA-seq) to compare gene expression in conjunctival immune cells. Conjunctival immune cells were immunophenotyped by flow cytometry and confocal microscopy. The activity of RXRalpha ligand 9-cis retinoic acid (RA) was evaluated in cultured monocytes and gammadelta T cells. RESULTS: Compared to wild type (WT) C57BL/6, Pinkie has increased signs of dry eye disease, including decreased tear volume, corneal barrier disruption, corneal/conjunctival cornification and goblet cell loss, and corneal vascularization, opacification, and ulceration with aging. ScRNA-seq of conjunctival immune cells identified gammadelta T cells as the predominant IL-17 expressing population in both strains and there is a 4-fold increased percentage of gammadelta T cells in Pinkie. Compared to WT, IL-17a, and IL-17f significantly increased in Pinkie with conventional T cells and gammadelta T cells as the major producers. Flow cytometry revealed an increased number of IL-17(+) gammadelta T cells in Pinkie. Tear concentration of the IL-17 inducer IL-23 is significantly higher in Pinkie. 9-cis RA treatment suppresses stimulated IL-17 production by gammadelta T and stimulatory activity of monocyte supernatant on gammadelta T cell IL-17 production. Compared to WT bone marrow chimeras, Pinkie chimeras have increased IL-17(+) gammadelta T cells in the conjunctiva after desiccating stress and anti-IL-17 treatment suppresses dry eye induced corneal MMP-9 production/activity and conjunctival goblet cell loss. CONCLUSION: These findings indicate that RXRalpha suppresses generation of dry eye disease-inducing IL-17 producing lymphocytes s in the conjunctiva and identifies RXRalpha as a potential therapeutic target in dry eye. |
