Primary Identifier | MGI:3612536 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Pkd1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An inverted mc1 promoter-driven neo flanked by two loxP sites was inserted into intron 34. A third loxP site was inserted into intron 30. RT-PCR detected a faint band. Wesetern blot detected a faint baind corresponding to amino acids 866-882. These analyses indicated that the insertion causes a significant disturbance but does not completely abrogate expresson of the gene. |