| First Author | Komori T | Year | 1993 |
| Journal | Science | Volume | 261 |
| Issue | 5125 | Pages | 1171-5 |
| PubMed ID | 8356451 | Mgi Jnum | J:75921 |
| Mgi Id | MGI:2178032 | Doi | 10.1126/science.8356451 |
| Citation | Komori T, et al. (1993) Lack of N regions in antigen receptor variable region genes of TdT-deficient lymphocytes. Science 261(5125):1171-5 |
| abstractText | During the assembly of immunoglobulin and T cell receptor variable region genes from variable (V), diversity (D), and joining (J) segments, the germline-encoded repertoire is further diversified by processes that include the template-independent addition of nucleotides (N regions) at gene segment junctions. Terminal deoxynucleotidyl transferase (TdT)-deficient lymphocytes had no N regions in their variable region genes, which shows that TdT is responsible for N region addition. In addition, certain variable region genes appeared at increased frequency in TdT-deficient thymocytes, which indicates that N region addition also influences repertoire development by alleviating sequence-specific constraints imposed on the joining of particular V, D, and J segments. |
