| Primary Identifier | MGI:3614440 | Allele Type | Transgenic |
| Attribute String | Inserted expressed sequence | Gene | Tg(CD2-Tgfbr2)1Grs |
| Inheritance Mode | Semidominant | Strain of Origin | C57BL/6 |
| Is Recombinase | false | Is Wild Type | false |
| description | This is a representative transgene. These data describe a composite of seven transgenic founders (T12I2, T12E1, T12E2, T12G2, T12H1, T12I3 and T12#9), five or six of which transmitted the transgene to their offspring. While the lines from which specific data are presented or illustrated are identified, it is unclear whether every test was performed on mice from every line. The phenotype is said to be qualitatively the same among different lines, but its age of onset, rate of progression and severity are reported to correlate with transgene copy number. (J:97523) |
| molecularNote | The transgene comprises a 0.7-kb DNA fragment encoding a truncated transforming growth factor beta type II receptor lacking the cytoplasmic tail, with a 5' influenza hemaglutinin (HA) tag, between a 5 kb human genomic DNA fragment containing the promoter through the first intron of the CD2 gene and a 5.5 kb fragment from the 3' untranslated region of human CD2 containing two polyadenylation signals and the locus control region (LCR). T-cell specific transgene mRNA expression in splenocytes of transgenic mice was demonstrated by semi-quantitative RT-PCR using primers for the HA tag sequence. Expression levels are similar in CD4+ and in CD8+ T cells. Transgene-derived, TGF beta 1-binding receptors were identified on T cells cultured from transgenic mice by incubating the cells with 125I-labeled ligand, then cross-linking the receptor-ligand complexes and precipitating them from cell lysates with HA tag-specific monoclonal antibodies. |
