| First Author | Chung SH | Year | 2013 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 54 |
| Issue | 10 | Pages | 6578-85 |
| PubMed ID | 23982838 | Mgi Jnum | J:214500 |
| Mgi Id | MGI:5603048 | Doi | 10.1167/iovs.13-12311 |
| Citation | Chung SH, et al. (2013) Laser capture microdissection-directed profiling of glycolytic and mTOR pathways in areas of selectively ablated Muller cells in the murine retina. Invest Ophthalmol Vis Sci 54(10):6578-85 |
| abstractText | PURPOSE: We have reported previously down-regulation of key metabolic pathways, the glycolytic and mTOR pathways, from a global retinal microarray analysis after selective Muller cell ablation in a novel transgenic model. The purpose of the present study was to examine changes in expression of key molecules of glycolytic and mTOR pathways specifically in patches of Muller cell loss. METHODS: Eyes were enucleated 1 and 3 months after induced Muller cell ablation, directly embedded in optimal cutting temperature medium, and snap frozen in liquid nitrogen. Laser capture microdissection (LCM) was conducted to dissect patches of Muller cell loss for quantitative RT-PCR (qRT-PCR) analysis of key genes of the glycolytic (glyceraldehyde-3-phosphate dehydrogenase, enolase 1 and 2, lactate dehydrogenase A and B) and mTOR pathways (insulin-like growth factor receptor 1, phosphatidylinositide-3-kinase, Akt1, and regulatory-associated protein of mTOR). Protein validations were performed by immunohistochemistry. RESULTS: The LCM-directed qRT-PCR analysis of Muller cell ablated specimens demonstrated reduced transcription of genes involved in the glycolytic and mTOR metabolic pathways. Of the proteins we chose to study, only enolase 1 was expressed by Muller cells. Other glycolytic and mTOR pathway proteins were expressed by photoreceptor inner and outer segments, which were lost in patches of Muller cell ablation. CONCLUSIONS: We found suppression of genes encoding various glycolytic and mTOR pathway-associated enzymes in areas of Muller cell loss. This appeared mainly to be due to loss of photoreceptor inner and outer segments. The consequences of metabolic derangement caused by Muller cell ablation warrant further investigation. |
