| Primary Identifier | MGI:3618371 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Lims1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Five days after retroviral Cre-mediated recombination homozygous floxed MEF cells retract but do not show increased apoptosis (J:105248). |
| molecularNote | Exon 4 was flanked with loxP sites. A floxed neo-tk cassette was removed in the ES cells during the generation of this allele. Cre mediated recombination removes exon 4 resulting in a frame shift and at most a short, non-functional peptide. Cre mediated recombination in ES of the parent allele (containing the floxed neo-tk and a third loxP site by exon 4) that removed both the neo-tk cassette and exon 4 produced Lims1tm2Ref. |
