Primary Identifier | MGI:3620008 | Allele Type | Targeted |
Attribute String | Conditional ready, No functional change | Gene | Braf |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting construct was designed to flank exon 12 (exon 9 according to nomenclature in J:29234) with loxP sites. This exon is the first to encode the kinase domain. Its excision would result in a frame shift in the ORF, resulting in a null mutation. Western blot confirmed that insertion of the loxP sites did not affect protein expression levels. |