| Primary Identifier | MGI:3619344 | Allele Type | Transgenic |
| Attribute String | Reporter | Gene | Tg(ACTB-ALPP)02Jrst |
| Strain of Origin | FVB/N | Is Recombinase | false |
| Is Wild Type | false |
| description | Histological analysis for heat-stable alkaline phosphatase activity identifies cells in which a deletional mutation has caused reversion of the mutant phenotype. |
| molecularNote | The transgene comprises a mutant human genomic placental alkaline phosphatase gene joined to the enhancer/promoter from the human beta-actin gene. The mutation was generated by inserting 7 G:C base pairs downstream of a series of 4 G:C pairs near the start of the reading frame, causing a frameshift that prevents production of a functional enzyme. Loss of a single base pair from the 11-G:C tract restores the reading frame and results in reversion to the wild-type enzymatic phenotype. |
