| Primary Identifier | MGI:3619527 | Allele Type | Targeted |
| Attribute String | Conditional ready, No functional change | Gene | Spi1 |
| Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
| Is Recombinase | false | Is Wild Type | false |
| description | Crossing mice bearing this "floxed" allele with mice expressing Cre recombinase under control of a selected promoter will result in excision of the URE in the cell/tissue type of interest. |
| molecularNote | This allele was generated by crossing mice bearing Sfpi1tm1Dgt with mice ubiquitously expressing FLP recombinase to produce progeny in which the FRT-flanked PGKneo cassette has been deleted. This leaves a single FRT site immediately upstream of the 5' member of a pair of loxP sites flanking the 3.4-kb upstream regulatory element (URE) that is located 14 kb upstream of the endogenous gene. Expression of the protein is unaffected by presence of the FRT or loxP sites. |
