| Primary Identifier | MGI:3623309 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Kmt2b |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Chimeras made using homozygous null ES cells where null cells are over 50% of the total cell population are absent by E18.5. Chimeras where null cells are less than 50% of the total cell population survive but the percentage of null cells decreases with age so that by E18.5 very few homozygous null cells are present, suggesting a cell autonomous defect. |
| molecularNote | A targeting vector was designed to insert an FRT flanked sequence containing the splice acceptor sequence from exon 2 of engrailed 2, EMCV IRES, a lacZ-neo cassette and SV40 polyA signal into intron 1. There was a loxP site on the 3' end and a second loxP site inserted into intron 2. RT-PCR confirmed that the cassette truncated the transcript before exon 2. A transcript encoding the first 121 amino acids of the locus fused to 40 frameshifted amino acids encoded by the second exon of engrailed 2 could be expressed. Transcript was not detected by RT-PCR of mutants. |
