| First Author | Gelfanov V | Year | 1996 |
| Journal | J Immunol | Volume | 156 |
| Issue | 1 | Pages | 35-41 |
| PubMed ID | 8598484 | Mgi Jnum | J:30238 |
| Mgi Id | MGI:77752 | Doi | 10.4049/jimmunol.156.1.35 |
| Citation | Gelfanov V, et al. (1996) Activated alpha beta-CD8+, but not alpha alpha-CD8+, TCR-alpha beta+ murine intestinal intraepithelial lymphocytes can mediate perforin-based cytotoxicity, whereas both subsets are active in Fas-based cytotoxicity. J Immunol 156(1):35-41 |
| abstractText | CD8 single-positive (CD8+) T cells in murine intestinal intraepithelial lymphocytes (iIEL) consist of alpha alpha-CD8+ and alpha beta-CD8+ subpopulations. Cytotoxicity represents an important function of peripheral CD8+ T cells, so we examined perforin-granzymebased and FAs-based cytotoxicity of activated CD8+ iIEL even when allospecific TCR were present on the iIEL, as demonstrated by using 2C TCR transgenic mice. On the other hand, both CD8+ iIEL appeared to lose their ability to perform perforin-based killing after activation through TCR because fresh cells lysed P815 cells coated with anti-TCR beta-chain (TCR-beta) mAb, whereas cells activated by plate-bound anti-TCR mAb did not. Of interest, both activated CD8+ TCR-alpha beta+ iIEL subsets, but not fresh cells, were able to mediate Fas-based killing when triggered with PMA and CA2+ ionophore. |
