Primary Identifier | MGI:3621477 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Gnasas1 |
Transmission | Germline | Strain of Origin | 129S/SvEv |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A targeting vector was designed to delete 1.6 kb of genomic sequence covering the promoter, exon 1 and 631 bp of intronic sequence, leaving the Gnas minimum promoter region intact. The vector included a tACE-Cre gene for excision of the cassette in the male germ line. RT-PCR failed to detect transcript in paternally inherited mutants. |