| First Author | Monroe RJ | Year | 1999 |
| Journal | Immunity | Volume | 11 |
| Issue | 2 | Pages | 201-12 |
| PubMed ID | 10485655 | Mgi Jnum | J:57268 |
| Mgi Id | MGI:1344158 | Doi | 10.1016/s1074-7613(00)80095-3 |
| Citation | Monroe RJ, et al. (1999) RAG2:GFP knockin mice reveal novel aspects of RAG2 expression in primary and peripheral lymphoid tissues. Immunity 11(2):201-12 |
| abstractText | We generated mice in which a functional RAG2:GFP fusion gene is knocked in to the endogenous RAG2 locus. In bone marrow and thymus, RAG2:GFP expression occurs in appropriate stages of developing B and T cells as well as in immature bone marrow IgM+ B cells. RAG2:GFP also is expressed in IgD+ B cells following cross-linking of IgM on immature IgM+ IgD+ B cells generated in vitro. RAG2:GFP expression is undetectable in most immature splenic B cells; however, in young RAG2:GFP mice, there are substantial numbers of splenic RAG2:GFP+ cells that mostly resemble pre-B cells. The latter population decreases in size with age but reappears following immunization of older RAG2:GFP mice. We discuss the implications of these findings for current models of receptor assembly and diversification. |
