| First Author | Kim S | Year | 2024 |
| Journal | Nat Cell Biol | PubMed ID | 39117796 |
| Mgi Jnum | J:353324 | Mgi Id | MGI:7712256 |
| Doi | 10.1038/s41556-024-01480-1 | Citation | Kim S, et al. (2024) TorsinA is essential for neuronal nuclear pore complex localization and maturation. Nat Cell Biol |
| abstractText | As lifelong interphase cells, neurons face an array of unique challenges. A key challenge is regulating nuclear pore complex (NPC) biogenesis and localization, the mechanisms of which are largely unknown. Here we identify neuronal maturation as a period of strongly upregulated NPC biogenesis. We demonstrate that the AAA+ protein torsinA, whose dysfunction causes the neurodevelopmental movement disorder DYT-TOR1A dystonia and co-ordinates NPC spatial organization without impacting total NPC density. We generated an endogenous Nup107-HaloTag mouse line to directly visualize NPC organization in developing neurons and find that torsinA is essential for proper NPC localization. In the absence of torsinA, the inner nuclear membrane buds excessively at sites of mislocalized nascent NPCs, and the formation of complete NPCs is delayed. Our work demonstrates that NPC spatial organization and number are independently determined and identifies NPC biogenesis as a process vulnerable to neurodevelopmental disease insults. |
