| First Author | Elgheznawy A | Year | 2023 |
| Journal | Front Immunol | Volume | 14 |
| Pages | 1197894 | PubMed ID | 37359521 |
| Mgi Jnum | J:337461 | Mgi Id | MGI:7494758 |
| Doi | 10.3389/fimmu.2023.1197894 | Citation | Elgheznawy A, et al. (2023) Loss of zinc transporters ZIP1 and ZIP3 augments platelet reactivity in response to thrombin and accelerates thrombus formation in vivo. Front Immunol 14:1197894 |
| abstractText | Zinc (Zn(2+)) is considered as important mediator of immune cell function, thrombosis and haemostasis. However, our understanding of the transport mechanisms that regulate Zn(2+) homeostasis in platelets is limited. Zn(2+) transporters, ZIPs and ZnTs, are widely expressed in eukaryotic cells. Using mice globally lacking ZIP1 and ZIP3 (ZIP1/3 DKO), our aim was to explore the potential role of these Zn(2+) transporters in maintaining platelet Zn(2+) homeostasis and in the regulation of platelet function. While ICP-MS measurements indicated unaltered overall Zn(2+) concentrations in platelets of ZIP1/3 DKO mice, we observed a significantly increased content of FluoZin3-stainable free Zn(2+), which, however, appears to be released less efficiently upon thrombin-stimulated platelet activation. On the functional level, ZIP1/3 DKO platelets exhibited a hyperactive response towards threshold concentrations of G protein-coupled receptor (GPCR) agonists, while immunoreceptor tyrosine-based activation motif (ITAM)-coupled receptor agonist signalling was unaffected. This resulted in enhanced platelet aggregation towards thrombin, bigger thrombus volume under flow ex vivo and faster in vivo thrombus formation in ZIP1/3 DKO mice. Molecularly, augmented GPCR responses were accompanied by enhanced Ca(2+) and PKC, CamKII and ERK1/2 signalling. The current study thereby identifies ZIP1 and ZIP3 as important regulators for the maintenance of platelet Zn(2+) homeostasis and function. |
