| First Author | Naramura M | Year | 1998 |
| Journal | Immunity | Volume | 9 |
| Issue | 2 | Pages | 209-16 |
| PubMed ID | 9729041 | Mgi Jnum | J:49411 |
| Mgi Id | MGI:1277456 | Doi | 10.1016/s1074-7613(00)80603-2 |
| Citation | Naramura M, et al. (1998) Mice with a fluorescent marker for interleukin 2 gene activation. Immunity 9(2):209-16 |
| abstractText | Production of interleukin (IL)-2 by T lymphocytes is one of the earliest events during immune response. A mutant mouse strain was generated by replacing the IL-2 gene with a cDNA encoding green fluorescent protein (GFP). In this model, GFP fluorescence is readily detectable upon T cell activation and is mostly coexpressed with IL-2 at the single cell level. Thus, individual activated T cells can express the IL-2 gene biallelically. Upon stimulation through the T cell antigen receptor, CD4+ cells separate into distinct GFP+ and GFP-populations, both of which are capable of differentiating into either Th1 or Th2 effectors. These mice allow noninvasive detection of IL-2 production by single cells and analysis of the subsequent differentiative fate of these cells as an immune response develops. |
