| Primary Identifier | MGI:3630292 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Cd8a |
| Transmission | Germline | Strain of Origin | 129S4/SvJae-Tg(Prm-cre)70Og |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | Two DNAaseI hypersensitive sites upstream of the Cd8a gene, HSS1 and HSS2, were deleted by homologous recombination. These sites are thought to contain regulatory sequences that control Cd8a expression. Targeting took place in ES cells homozygous for the Tg(Prm-cre)70Og transgene, which expresses Cre recombinase in male germ cells. An inserted loxP-flanked neomycin selection cassette was deleted by Cre-mediated recombination upon transmission from male chimeric mice. |
